AI RESEARCH
Segmentation-before-Staining Improves Structural Fidelity in Virtual IHC-to-Multiplex IF Translation
arXiv CS.CV
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ArXi:2603.16160v1 Announce Type: new Multiplex immunofluorescence (mIF) enables simultaneous single-cell quantification of multiple biomarkers within intact tissue architecture, yet its high reagent cost, multi-round staining protocols, and need for specialized imaging platforms limit routine clinical adoption. Virtual staining can synthesize mIF channels from widely available brightfield immunohistochemistry (IHC), but current translators optimize pixel-level fidelity without explicitly cons.